| Cat. No. : |
IL11-218H |
| Description : |
Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation. It is also known under the names Adipogenesis inhibitory factor (AGIF) and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis. |
| Source : |
Sf21 Insect Cells. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Human IL-11 is Mr 19 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 23 kDa. |
| State Of Matter : |
Solution. |
| Formulation : |
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present. The final protein concentration is 0.25 mg/ml. |
| Purity : |
> 97% by SDS Page and analyzed by silver stain. |
| Endotoxin : |
< 0.1 ng per 1 µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Human IL-11 was determined by a cell proliferation assay using T11, a subline of the IL-6-dependent murine plasmacytoma cell line T1165.85.2.1 that has been adapted to grow in IL-11. The expected ED50 for this effect is typically 0.06 - 0.24 ng/mL. |
| Storage And Stability : |
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20˚C to -70˚C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2˚C - 8˚C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20˚C to -70˚C in a manual defrost freezer. Avoid repeated freeze thaw cycles. |
