||Recombinant Human NTRK1 encoding the signal peptide from human CD33 joined with the extracellular domain of human Trk A (Ala 33 - Glu 407) was fused to the Fc region of human IgG1 via a polypeptide linker and expressed inNSO cells.
||This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer.
||The predicted molecular weight of Recombinant Human is Mr 67.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 115-125 kDa.
|State Of Matter:
||This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
||>95% by SDS Page and analyzed by silver stain.
||The biological activity of Human TrkA was determined by its ability to inhibit NGF-induced proliferation of TF1 cells. The ED50 for this effect is typically 0.8 - 4 μg/mL in the presence of 10 ng/mL of rhNGF.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.