Recombinant Human PCK1 protein, GST-tagged

• Cat.No.: PCK1-30553TH
• Product Overview: Recombinant Human PCK1(1 a.a. - 622 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 94.16 kDa
• AA Sequence: MPPQLQNGLNLSAKVVQGSLDSLPQ AVREFLENNAELCQPDHIHICDGSE EENGRLLGQMEEEGILRRLKKYDNC WLALTDPRDVARIESKTVIVTQEQR DTVPIPKTGLSQLGRWMSEEDFEKA FNARFPGCMKGRTMYVIPFSMGPLG SPLSKIGIELTDSPYVVASMRIMTR MGTPVLEALGDGEFVKCLHSVGCPL PLQKPLVNNWPCNPELTLIAHLPDR REIISFGSGYGGNSLLGKKCFALRM ASRLAKEEGWLAEHMLVLGITNPEG EKKYLAAAFPSACGKTNLAMMNPSL PGWKVECVGDDIAWMKFDAQGHLRA INPENGFFGVAPGTSVKTNPNAIKT IQKNTIFTNVAETSDGGVYWEGIDE PLASGVTITSWKNKEWSSEDGEPCA HPNSRFCTPASQCPIIDAAWESPEG VPIEGIIFGGRRPAGVPLVYEALSW QHGVFVGAAMRSEATAAAEHKGKII MHDPFAMRPFFGYNFGKYLAHWLSM AQHPAAKLPKIFHVNWFRKDKEGKF LWPGFGENSRVLEWMFNRIDGKAST KLTPIGYIPKEDALNLKGLGHINMM ELFSISKEFWEKEVEDIEKYLEDQV NADLPCEIEREILALKQRISQM
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ]
• Official Symbol: PCK1
• Synonyms: PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK C; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase; phosphoenolpyruvate carboxykinase, cytosolic; PEPCK1; PEPCKC; PEPCK-C; MGC22652
• Gene ID: 5105
• mRNA Refseq: NM_002591
• Protein Refseq: NP_002582
• MIM: 614168
• UniProt ID: P35558
• Chromosome Location: 20q13.31
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem
• Function: GDP binding; GTP binding; carboxylic acid binding; lyase activity; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; purine nucleotide binding