Recombinant Human YARS, His-tagged

• Cat.No.: YARS-31445TH
• Product Overview: Recombinant full length Human Tyrosyl tRNA synthetase with an N terminal His tag ; predicted mwt: 61.3 kDa

Specification

• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
• Protein length: 528 amino acids
• Conjugation: HIS
• Molecular Weight: 61.300kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGDAP SPEEKLHLITRNLQEVLGEEKLKEI LKERELKIYWGTATTGKPHVAYFVP MSKIADFLKAGCEVTILFADLHAYL DNMKAPWELLELRVSYYENVIKAML ESIGVPLEKLKFIKGTDYQLSKEYT LDVYRLSSVVTQHDSKKAGAEVVKQ VEHPLLSGLLYPGLQALDEEYLKVD AQFGGIDQRKIFTFAEKYLPALGYS KRVHLMNPMVPGLTGSKMSSSEEES KIDLLDRKEDVKKKLKKAFCEPGNV ENNGVLSFIKHVLFPLKSEFVILRD EKWGGNKTYTAYVDLEKDFAAEVVH PGDLKNSVEVALNKLLDPIREKFNT PALKKLASAAYPDPSKQKPMAKGPA KNSEPEEVIPSRLDIRVGKIITVEK HPDADSLYVEKIDVGEAEPRTVVSG LVQFVPKEELQDRLVVVLCNLKPQK MRGVESQGMLLCASIEGINRQVEPL DPPAGSAPGEHVFVKGYEKGQPDEE LKPKKKVFEKLQADFKISEECIAQW KQTNFMTKLGSISCKSLKGGNIS
• Sequence Similarities: Belongs to the class-I aminoacyl-tRNA synthetase family.Contains 1 tRNA-binding domain.

Gene Information

• Gene Name: YARS tyrosyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: YARS
• Synonyms: YARS; tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; tyrosine tRNA ligase 1; cytoplasmic; tyrRS; YRS; YTS
• Gene ID: 8565
• mRNA Refseq: NM_003680
• Protein Refseq: NP_003671
• MIM: 603623
• Uniprot ID: P54577
• Chromosome Location: 1p35.1
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; interleukin-8 receptor binding; ligase activity; nucleotide binding; signal transducer activity