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Recombinant Human Hexokinase 2, His-tagged

Cat. No.: HK2-41H
Product Overview: Recombinant human Hexokinase2, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 104.1kDa (937aa).
Description: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR
Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Purity: > 95% by SDS - PAGE
Concentration: 1 mg/ml (determined by Bradford assay)
Biological activity: Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30°C.
Activity Assay: 1.Prepare a 2.57ml reaction mixture into a suitable container: The final concentrations are 39 mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD.2.Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3.Add 5ug of recombinant hexokinase1 into reaction mixture and mix immediately. 4.Record the increase in A340nm for 5 minutes.
Storagee: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
GeneIDD: 3099
Gene Name:
Synonyms: Hexokinase-2; EC 2.7.1.1; HK2; Hexokinase type II; HK II; Muscle form hexokinase; HXK2; DKFZp686M1669; Muscle form hexokinase; hexokinase 2; hexokinase-2, muscle; HKII
mRNA Refseq: NM_000189
Protein Refseq: NP_000180
MIM: 601125
UniProt ID: P52789
Chromosome Location: 2p13
Pathway: Aminosugars metabolism; Fructose and mannose metabolism; Galactose metabolism; Glycolysis / Gluconeogenesis; Starch and sucrose metabolism; Streptomycin biosynthesis; Metabolism of carbohydrates
Function: ATP binding; hexokinase activity; kinase activity; nucleotide binding; transferase activity

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