Recombinant Full Length Human HK2 Protein, C-Flag-tagged

• Cat.No.: HK2-359HFL
• Product Overview: Recombinant Full Length Human HK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 102.2 kDa
• AA Sequence: MIASHLLAYFFTELNHDQVQKVDQY LYHMRLSDETLLEISKRFRKEMEKG LGATTHPTAAVKMLPTFVRS TPDGTEHGEFLALDLGGTNFRVLWV KVTDNGLQKVEMENQIYAIPEDIMR GSGTQLFDHIAECLANFMDK LQIKDKKLPLGFTFSFPCHQTKLDE SFLVSWTKGFKSSGVEGRDVVALIR KAIQRRGDFDIDIVAVVNDT VGTMMTCGYDDHNCEIGLIVGTGSN ACYMEEMRHIDMVEGDEGRMCINME WGAFGDDGSLNDIRTEFDQE IDMGSLNPGKQLFEKMISGMYMGEL VRLILVKMAKEELLFGGKLSPELLN TGRFETKDISDIEGEKDGIR KAREVLMRLGLDPTQEDCVATHRIC QIVSTRSASLCAATLAAVLQRIKEN KGEERLRSTIGVDGSVYKKH PHFAKRLHKTVRRLVPGCDVRFLRS EDGSGKGAAMVTAVAYRLADQHRAR QKTLEHLQLSHDQLLEVKRR MKVEMERGLSKETHASAPVKMLPTY VCATPDGTEKGDFLALDLGGTNFRV LLVRVRNGKWGGVEMHNKIY AIPQEVMHGTGDELFDHIVQCIADF LEYMGMKGVSLPLGFTFSFPCQQNS LDESILLKWTKGFKASGCEG EDVVTLLKEAIHRREEFDLDVVAVV NDTVGTMMTCGFEDPHCEVGLIVGT GSNACYMEEMRNVELVEGEE GRMCVNMEWGAFGDNGCLDDFRTEF DVAVDELSLNPGKQRFEKMISGMYL GEIVRNILIDFTKRGLLFRG RISERLKTRGIFETKFLSQIESDCL ALLQVRAILQHLGLESTCDDSIIVK EVCTVVARRAAQLCGAGMAA VVDRIRENRGLDALKVTVGVDGTLY KLHPHFAKVMHETVKDLAPKCDVSF LQSEDGSGKGAALITAVACR
  IREAGQRTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus

Gene Information

• Gene Name: HK2 hexokinase 2 [ Homo sapiens (human) ]
• Official Symbol: HK2
• Synonyms: HKII; HXK2
• Gene ID: 3099
• mRNA Refseq: NM_000189.5
• Protein Refseq: NP_000180.2
• MIM: 601125
• UniProt ID: P52789