Recombinant Human Mammaglobin

• Cat.No.: MGB2-158H
• Product Overview: Recombinant Human Mammaglobin protein has a total sequence of 87 AA. MW: 9.88 kDa (calculated). N-Terminal His-tag (12 extra AA)

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The mammaglobin gene was first identified using a differential screening approach directed at the isolation of novel, human breast cancer-associated genes. Mammaglobin encodes a 10 kDa glycoprotein and is distantly relaetd to a family of epithelial secretory proteins that includes rat estramustine-binding protein/prostatein and human Clara cell 10 kDa protein (CC10)/uteroglobin. Mammaglobin, a mammary-specific member of the uterglobin family, is known to be overexpressed in human breast cancer. Studies suggest that mammaglobin is one of the first relatively mammary-specific and mammary-sensitive markers (85%). Mammaglobin may be valuable used in a panel with BRST-2 (GCDFP-15) and ER in evaluating tumors of unknown primary sites.
• Form: Filtered (0,4 m) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.05 M NaCl, pH 7.2.
• Molecular Mass: 9.88 kDa
• AA Sequence: MRGSHHHHHH GSGSGCPLLE NVISKTINPQ VSKTEYKELL QEFIDDNATT NAIDELKECF LNQTDETLSN VEVFMQLIYD SSLCDLF
• Endotoxin: <1.0 EU/ug
• Purity: Purity as determined by densitometric image analysis: 90%.
• Quality Control Test: BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein. LAL to determine quantity of endotoxin.
• Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Publications:
  Development of quantitative immunochromatographic assay for rapid and sensitive detection of carbohydrate antigen 19-9 (CA 19-9) in human plasma (2017)
  Development of Quantitative Lateral Flow Strip Biosensors for the Detection of Cancer Biomarkers (2019)
  Carbon nanotube-based lateral flow immunoassay for ultrasensitive detection of proteins: application to the determination of IgG (2019)

Citation

Carbon nanotube-based lateral flow immunoassay for ultrasensitive detection of proteins: application to the determination of IgG.

Journal: Mikrochimica acta    PubMed ID: 31197469    Data: 2020/4/6

Authors: Wanwei Qiu, Kwaku Baryeh, Guodong Liu

Article Snippet:Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19–9) were obtained from Fitzgerald Industries International (Acton, MA, USA, https://www.fitzgerald-fii. com/).Carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19–9) were obtained from Fitzgerald Industries International (Acton, MA, USA, https://www.fitzgerald-fii. com/).. Human mammaglobin was purchased from Creative BioMart (Shirley, NY, USA, https://www.creativebiomart. net/).. N-(3-Dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfoNHS), 2-(4- Morpholino) ethanesulfonic acid (MES), tween 20, triton X-100, bovine serum albumin (BSA), sucrose, polyvinylpyrrolidone (PVP), phosphate buffered saline (0.N-(3-Dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfoNHS), 2-(4- Morpholino) ethanesulfonic acid (MES), tween 20, triton X-100, bovine serum albumin (BSA), sucrose, polyvinylpyrrolidone (PVP), phosphate buffered saline (0.

Development of Quantitative Immunochromatographic Assay for Rapid and Sensitive Detection of Carbohydrate Antigen 19-9 (CA 19-9) in Human Plasma

Journal: Journal of pharmaceutical and biomedical analysis    PubMed ID: 28898765    Data: 2017/9/5

Authors: Kwaku Baryeh, Sunitha Takalkar, Guodong Liu

Article Snippet:The anti-CA 19-9 antibodies were designated as anti-CA 19-9 AbA and anti-CA 19-9 AbB, respectively.The anti-CA 19-9 antibodies were designated as anti-CA 19-9 AbA and anti-CA 19-9 AbB, respectively.. Human mammaglobin was purchased from Creative BioMart (Shirley, NY, USA).. CA 19-9 ELISA kit (EHCA 19-9) and goat anti-mouse IgG ( {"type":"entrez-nucleotide","attrs":{"text":"A16092","term_id":"492990"}} A16092 ) was purchased from Thermofisher Scientific (Waltham, MA, USA).CA 19-9 ELISA kit (EHCA 19-9) and goat anti-mouse IgG ( {"type":"entrez-nucleotide","attrs":{"text":"A16092","term_id":"492990"}} A16092 ) was purchased from Thermofisher Scientific (Waltham, MA, USA).

Selectivity of the developed GNP-based LFSB (concentration of CA 19-9 was 50 U mL?1, Mammaglobin, Human IgG and CEA were at 100 ng mL?1).