Recombinant Human Aurora Kinase A, His-tagged

• Cat.No.: AURKA-549H
• Product Overview: Recombinant full-length human AURORA A was expressed bybaculovirus in Sf9 cellsusing an N-terminal His tag. MW = 50kDa.

Specification

• Cat. No.: AURKA-549H
• Description: AURORA A belongs to a multigenic family of mitotic serine/threonine kinases which are involved in the control of chromosome segregation. AURORA A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. AURORA A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of AURORA A has been found to be correlated with the grade of various human solid tumours. Ectopic AURORA A overexpression in any culture cell line leads to polyploidy and centrosome amplification.
• Source: Baculovirus infected Sf9 cells.
• Purity: >90% by SDS-PAGE.
• Specific Activity: 120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM aurora A substrate, and 0.4 ug/ml Aurora A kinase.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: AURKA aurora kinase A [ Homo sapiens ]
• Synonyms: AURKA ; aurora kinase A; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; OTTHUMP00000031344; OTTHUMP00000166071; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; breast-tumor-amplified kinase; serine/threonine protein kinase 15; EC 2.7.11.1; AurA; Aurora kinase A; Aurora-A; Serine/threonine kinase 15; hARK1; Breast tumor-amplified kinase; OTTHUMP00000031340; OTTHUMP00000031342; OTTHUMP00000031343; OTTHUMP00000031345; Breast tumor-amplified kinase 3; serine/threonine protein kinase 6
• Gene ID: 6790
• mRNA Refseq: NM_003600
• Protein Refseq: NP_003591
• MIM: 603072
• UniProt ID: O14965
• Chromosome Location: 20q13
• Pathway: Cell Cycle, Mitotic
• Function: ATP binding; nucleotide binding; protein binding; protein binding; protein serine/threonine kinase activity; transferase activity; ubiquitin protein ligase binding