||Recombinant Human CYP2C9 allele expressed ininsect cellsis the most common in human populations studied to date and contains Arg, Ile and Gly at positions 144, 359 and 475 respectively.
||CYP2C9 is an important cytochrome P450 enzyme with a major role in the oxidation of both xenobiotic and endogenous compounds. CYP2C9 makes up about 18% of the cytochrome P450 protein in liver microsomes. Some 100 therapeutic drugs are metabolized by CYP2C9, including drugs with a narrow therapeutic index such as warfarin and phenytoin and other routinely prescribed drugs such as acenocoumarol, tolbutamide, losartan, glipizide, and some nonsteroidal anti-inflammatory drugs.
||5.5 mg/mL as determined using the BCA assay. Buffer: 100 mM potassium phosphate (pH 7.5), 20% (v/v) glycerol, 0.1 mM EDTA, 1 mM DTT.
||200 pmol spectral P450 per milligram of protein Cytochrome.
||1.19 μmol of cytochrome c reduced per minute per milligram of protein.
||6.6+0.881 nmol/min/mg of protein. Diclofenac hydroxylation reactions were performed for 10 minutes at 37°C. Reactions were carried out in the presence of 0.1 M Tris HCl (pH 7.5), 100 μM diclofenac, 2 mM NADPH, and at a final protein concentration of 100 μg/mL. The reactions were stopped by addition of 20 μL acetonitrile/glacial acetic acid (96:4).
||−80℃; Avoid multiple freeze-thaws.
||Normal precautions exercised in handling laboratory reagents should be followed. The chemical, physical, and toxicological properties of these products may not, as yet, have been thoroughly investigated. We recommend the use of gloves, lab coats, and eye protection when working with any chemical reagents.