Recombinant Yeast TRR1
| Cat.No. : | TRR1-1907Y |
| Product Overview : | Recombinant Yeast Thioreduxin Reductase produced inE.coliis a single, non-glycosylated, polypeptide chain, 36kDa. |
- Specification
- Gene Information
- Related Products
| Cat. No. : | TRR1-1907Y |
| Source : | E.coli. |
| Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val. |
| Form : | Purified, in PBS, pH 7.4. |
| Purity : | >95%, as determined by: (a) Analysis by RP-HPLC and anion-exchange FPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Dimers & Aggregates : | Less than 1% as determined by silver stained SDS-PAGE gel analysis. |
| Endotoxin : | Less than 0.1 ng/µg (IEU/µg) of ryNTR. |
| Protein Content : | Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of CTLA-4 as a Reference Standard. |
| Application : | 1) Molecular standard (Western, ELISA) in studying secreted BMP-4. 2) Preparing antibodies for BMP-4 monomer. 3) Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE. |
| Biological Activity : | RyNTR is fully biologically active when compared to standardt Definition: One unit equals the change in absorbance at 412 nm per ute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0) specific activity was found to be 5IU/mg. |
| Storage : | -20°C (aliquot), avoid freezing and thawing cycles. |
| Pathways : | Pyrimidine metabolism |
| Gene Name : | TRR1 Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress [ Saccharomyces cerevisiae ] |
| Synonyms : | Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; TRR1;Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress Trr1p |
| Gene ID : | 851955 |
| Protein Refseq : | NP_010640 |
| Chromosome Location : | IV |
| Function : | FAD binding; ferrous iron binding; oxidoreductase activity; thioredoxin-disulfide reductase activity |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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