||Recombinant Human phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) is expressed inE. coliwith N-terminal His-tagged. The molecular weight of the recombinant human PLA2G7 is 46.4 kDa.
||Platelet-activating factor acetylhydrolase (PAF-AH) is an extracellular phospholipase A2 which hydrolyzes the acetyl group at the sn-2 position of phospholipids. Two main types of PAF-AH have been characterized, namely the secreted (i.e. plasma) and intracellular enzymes, which are encoded on individual genes and share only 41% homology at the amino acid level. PAF deacetylation by PAF-AH causes loss of biological activity, making PAF-AH potential useful as an anti-inflammation therapy. Human recombinant PAF-AH was characterized using 2-Thio PAF as a substrate, which upon hydrolysis by PAF-AH releases a free thiol that reacts with 5,5-dithio-bis(2-dinitrobenzoic acid) (DTNB) to produce 5-thio-2-nitrobenzoic acid (TNB) with an absorbance maximum at 412 nm.
||Greater than 95% as determined by SDS-PAGE.
||100 mM Sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
||-80℃. 1 year.