Recombinant Human PLA2G7, His-tagged

• Cat.No.: PLA2G7-581H
• Product Overview: Recombinant Human phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) is expressed inE. coliwith N-terminal His-tagged. The molecular weight of the recombinant human PLA2G7 is 46.4 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Platelet-activating factor acetylhydrolase (PAF-AH) is an extracellular phospholipase A2 which hydrolyzes the acetyl group at the sn-2 position of phospholipids. Two main types of PAF-AH have been characterized, namely the secreted (i.e. plasma) and intracellular enzymes, which are encoded on individual genes and share only 41% homology at the amino acid level. PAF deacetylation by PAF-AH causes loss of biological activity, making PAF-AH potential useful as an anti-inflammation therapy. Human recombinant PAF-AH was characterized using 2-Thio PAF as a substrate, which upon hydrolysis by PAF-AH releases a free thiol that reacts with 5,5-dithio-bis(2-dinitrobenzoic acid) (DTNB) to produce 5-thio-2-nitrobenzoic acid (TNB) with an absorbance maximum at 412 nm.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: 100 mM Sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
• Storage: -80℃. 1 year.

Gene Information

• Gene Name: PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) [ Homo sapiens ]
• Synonyms: PLA2G7; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); LP-PLA2; Platelet-Activating Factor Acetylhydrolase; PAF-AH;Lipoprotein PLA2; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAFAH; lipoprotein-associated phospholipase A2; LDL-PLA2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase
• Gene ID: 7941
• mRNA Refseq: NM_001168357
• Protein Refseq: NP_001161829
• MIM: 601690
• UniProt ID: Q13093
• Chromosome Location: 6p21.2-p12
• Pathway: Ether lipid metabolism; Metabolic pathways; Diabetes pathways
• Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; hydrolase activity; phospholipid binding