Recombinant Human CADM3 protein, His-tagged CADM3-2184H

Recombinant Human CADM3 protein, His-tagged

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Recombinant Human CADM3 protein, His-tagged

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Cat.No. : CADM3-2184H
Product Overview : Recombinant Human CADM3 (Asn25-His330) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Cell adhesion molecule 3 (CADM3) is also known as Synaptic cell adhesion molecule 3 (SynCAM3), Immunoglobulin superfamily member 4B (IgSF4B), Brain immunoglobulin receptor (BIgR), Nectin-like protein 1 (NECL-1), TSLC1-like protein 1 (TSLL1), which belongs to the nectin family. CADM3 contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain.The isoform 1 is expressed mainly in adult and fetal brain and isoform 2 is highly expressed in adult brain and weakly expressed in placenta. In brain, Isoform 2 is highly expressed in cerebellum. CADM3 involved in the cell-cell adhesion and has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. CADM3 / SynCAM3 interaction with EPB41L1 may regulate structure or function of cell-cell junctions.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Asn25
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 34.5 kDa. The protein migrates as 38-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Asn25-His330
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CADM3-2184H :

Human CADM3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Gene Name : CADM3
Official Symbol : CADM3
Synonyms : CADM3; cell adhesion molecule 3; IGSF4B, immunoglobulin superfamily, member 4B; BIgR; FLJ10698; Necl 1; NECL1; nectin like 1; SynCAM3; TSLL1; TSLC1-like 1; nectin-like 1; TSLC1-like protein 1; nectin-like protein 1; brain immunoglobulin receptor; synaptic cell adhesion molecule 3; immunoglobulin superfamily member 4B; immunoglobulin superfamily, member 4B; dendritic cell nectin-like protein 1 short isoform; IGSF4B; Necl-1; synCAM3
Gene ID : 57863
mRNA Refseq : NM_001127173
Protein Refseq : NP_001120645
MIM : 609743
UniProt ID : Q8N126

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