Active Recombinant Human ACVR1B protein, His-tagged

• Cat.No.: ACVR1B-645H
• Product Overview: Recombinant Human ACVR1B protein(Ser 24 - Glu 126), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ser 24 - Glu 126
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human TDGF1, Fc Tag on Protein A Biosensor, can bind Human Activin RIB, His Tag with an affinity constant of 5.6 μM as determined in BLI assay (ForteBio Octet Red96e) (QC Tested).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 13.3 kDa. The protein migrates as 16-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Sulforaphane Suppresses the Growth of Triple-negative Breast Cancer Stem-like Cells In vitro and In vivo (2019)
  Human Cripto-1 and Cripto-3 Protein Expression in Normal and Malignant Settings That Conflicts with Established Conventions (2024)

Gene Information

• Gene Name: ACVR1B activin A receptor, type IB [ Homo sapiens ]
• Official Symbol: ACVR1B
• Synonyms: ACVR1B; activin A receptor, type IB; ACVRLK4; activin receptor type-1B; ActRIB; ALK4; SKR2; activin receptor-like kinase 4; activin A receptor, type II-like kinase 4; serine/threonine-protein kinase receptor R2; ACTRIB
• Gene ID: 91
• mRNA Refseq: NM_004302
• Protein Refseq: NP_004293
• MIM: 601300
• UniProt ID: P36896