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Recombinant Human Protein Kinase C, Alpha

Cat.No. : PRKCA-648H
Product Overview : Recombinant Human PRKCA is produced inBaculoviral insect expression system.
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  • Gene Information
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Cat. No. : PRKCA-648H
Description : MP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Source : Baculoviral insect expression system.
Format : Purified, Liquid.
Concentration : 20ug/ml.
Buffer : Suspended in SDS/mercaptoethanol sample buffer with a bromophenol blue dye marker.
Applications : Suitable for use as a standard in Western blot using enhanced chemiluminescence. Load 2-5ul directly from vial (no dilution necessary). For blots using less sensitive substrates, such as alkaline phosphatase, larger amount of protein should be loaded onto the gel. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Inactivation : Not applicable.
Important Note : Centrifuge before opening to ensure complete recovery of vial contents.
Storage : Short term (up to 1 year) store at –20°C. Long term, store at –70°C.
Gene Name : PRKCA protein kinase C, alpha [ Homo sapiens ]
Synonyms : PRKCA; protein kinase C, alpha; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha; EC 2.7.11. 13; Protein kinase C alpha type; aging-associated gene 6; protein kinase C alpha type
Gene ID : 5578
mRNA Refseq : NM_002737
Protein Refseq : NP_002728
MIM : 176960
UniProt ID : P17252
Chromosome Location : 17q22-q24
Pathway : Calcium signaling pathway; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; GnRH signaling pathway; Leukocyte transendothelial migration; Long-term potentiation; Long-term depression; MAPK signaling pathway; Melanogenesis; Natural killer cell mediated cytotoxicity; Non-small cell lung cancer; Pathogenic Escherichia coli infection - EHEC; Pathways in cancer; Phosphatidylinositol signaling system; Tight junction; VEGF signaling pathway; Vascular smooth muscle contraction; Vibrio cholerae infection; Wnt signaling pathway; Diabetes pathways; Hemostasis; Synaptic Transmission
Function : ATP binding; calcium ion binding; calcium-dependent protein kinase C activity; nucleotide binding; protein binding; transferase activity; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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03/12/2020

    When utilized in Western Blotting experiments, the PRKCA protein has consistently produced distinct and well-defined protein bands, facilitating clear and accurate interpretation of results.

    10/16/2017

      I have successfully implemented the PRKCA protein in protein electron microscopy structure analysis, where its attributes have greatly aided in elucidating complex molecular structures.

      01/09/2016

        Considering its remarkable performance across multiple assays, I wholeheartedly endorse the usage of the PRKCA protein in various research studies.

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