Recombinant Human Aurora Kinase A, His-tagged

• Cat.No.: AURKA-683H
• Product Overview: Recombinant human Aurora A kinase (amino acids 2-403) fused at the N-terminus to a His Tag sequence was expressed inS. frugiperdainsect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division. MW = 50 kDa.

Specification

• Cat. No.: AURKA-683H
• Description: Aurora A kinase also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. Aurora A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle.
• Source: S. frugiperda.
• Form: Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween-20 Detergent, pH 7.5. Please refer to vial label for lot-specific concentration.
• Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
• Purity: ≥90% by SDS-PAGE.
• Specific Activity: ≥120 U/µg protein.
• Toxicity: Regulatory Review.
• Storage: ≤ -70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: AURKA aurora kinase A [ Homo sapiens ]
• Synonyms:
  aurora kinase A; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; AURKA; serine/threonine-protein kinase 6; ARK-1; hARK1; OTTHUMP00000031341; OTTHUMP00000166072; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase aurora-A; Aurora/IPL1-related kinase 1; Serine/threonine-protein kinase aurora-A; Aurora-related kinase 1; Serine/threonine-protein kinase 15; Breast tumor-amplified kinase; EC 2.7.11.1; AurA
• Gene ID: 6790
• mRNA Refseq: NM_003600
• Protein Refseq: NP_003591
• MIM: 603072
• UniProt ID: O14965
• Chromosome Location: 20q13.2-q13.3
• Pathway: Oocyte meiosis; Cell Cycle, Mitotic
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; transferase activity; ubiquitin protein ligase binding