|Cat. No. :
|Product Overview :
||Recombinant human Aurora A kinase (amino acids 2-403) fused at the N-terminus to a His Tag sequence was expressed inS. frugiperdainsect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division. MW = 50 kDa.
||Aurora A kinase also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. Aurora A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle.
||Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween-20 Detergent, pH 7.5. Please refer to vial label for lot-specific concentration.
|Unit Definition :
||One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
||≥90% by SDS-PAGE.
|Specific Activity :
||≥120 U/µg protein.
||≤ -70°C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).