||Protein Marker, Middle Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 6 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250.
||40mM Tris-HCl, pH6.8, 33mM NaCl, 5mM BME, 6M Urea, 10% Glycerol, 0.02% Bromophenol Blue, 2% SDS (w/v). Store at -20℃.
||1. Bring desired amount of the Protein marker over to a separate tube. For visualizing during electrophoresis: use 5 μl for mini-gels and 10 μl for full length gels. 2. The Marker is treated before packing. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.