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Recombinant Human Adenosylhomocysteinase, His-tagged

Cat. No.: AHCY-727H
Product Overview: Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =49.8 kDa (452aa).
Description: AHCY, also known as adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). It regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia.
Source: E.coli.
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVET AVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTG VHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMS CDGPFKPDHY RY
Purity: > 95% by SDS – PAGE.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
Concentration: 1.0 mg/ml (determined by Bradford assay).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens ]
Synonyms: AHCY; adenosylhomocysteinase; SAHH; S-adenosylhomocysteine hydrolase; EC 3.3.1.1; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; OTTHUMP00000030684
Gene ID: 191
mRNA Refseq: NM_000687
Protein Refseq: NP_000678
MIM: 180960
UniProt ID: P23526
Chromosome Location: 20cen-q13.1
Pathway: Methionine metabolism; Selenoamino acid metabolism; Biological oxidations
Function: adenosylhomocysteinase activity; copper ion binding; hydrolase activity; protein self-association

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