Active Recombinant Human AHCY Protein, 1-432aa, N-His tagged

• Cat.No.: AHCY-727H
• Product Overview: Recombinant human AHCY protein (1-432aa), fused to His-tag at N-terminus, was expressed in E. coli, and purified by using proprietary chromatography techniques. This enzyme is validated for AHCY activity application.

Specification

• Description: Adenosylhomocysteinase (AHCY) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine (Ado) and L-homocysteine (Hcy). It regulates the intracellular SAH concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia.
• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 1-432aa
• Form: Liquid
• Bio-activity: Specific activity is > 300 pmol/min/mg.
• Molecular Mass: 49.8 kDa (452aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMSDKL PYKVADIGLAAWGRKALDIAENEMP GLMRMRERYSASKPLKGARIAGCLH MTVETAVLIETLVTLGAEVQWSSCN IFSTQDHAAAAIAKAGIPVYAWKGE TDEEYLWCIEQTLYFKDGPLNMILD DGGDLTNLIHTKYPQLLPGIRGISE ETTTGVHNLYKMMANGILKVPAINV NDSVTKSKFDNLYGCRESLIDGIKR ATDVMIAGKVAVVAGYGDVGKGCAQ ALRGFGARVIITEIDPINALQAAME GYEVTTMDEACQEGNIFVTTTGCID IILGRHFEQMKDDAIVCNIGHFDVE IDVKWLNENAVEKVNIKPQVDRYRL KNGRRIILLAEGRLVNLGCAMGHPS FVMSNSFTNQVMAQIELWTHPDKYP VGVHFLPKKLDEAVAEAHLGKLNVK LTKLTEKQAQYLGMSCDGPFKPDHY RY.
• Purity: > 95% by SDS-PAGE
• Applications: Enzyme Activity, and protein assay by SDS-PAGE
• Storage: Store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay).
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, No DTT.

Gene Information

• Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens (human) ]
• Official Symbol: AHCY
• Synonyms: AHCY; adenosylhomocysteinase; S adenosylhomocysteine hydrolase; SAHH; adoHcyase; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase
• Gene ID: 191
• mRNA Refseq: NM_000687
• Protein Refseq: NP_000678
• MIM: 180960
• UniProt ID: P23526