Recombinant Human UDP-glucose 6-dehydrogenase, His-tagged

• Cat.No.: UGDH-729H
• Product Overview: Recombinant humanUGDH protein, fused to his-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 59.5 kDa (533aa).

Specification

• Cat. No.: UGDH-729H
• Description: UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver.This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate.These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.
• Source: E.coli.
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UGDH UDP-glucose 6-dehydrogenase [ Homo sapiens ]
• Synonyms: UGDH; UDP-glucose 6-dehydrogenase; GDH; UGD; UDPGDH; UDP-GlcDH; UDP-Glc dehydrogenase; UDP-glucose dehydrogenase; uridine diphospho-glucose dehydrogenase; EC 1.1.1.22
• Gene ID: 7358
• mRNA Refseq: NM_001184700
• Protein Refseq: NP_001171629
• MIM: 603370
• UniProt ID: O60701
• Chromosome Location: 4p15.1
• Pathway: Amino sugar and nucleotide sugar metabolism; Ascorbate and aldarate metabolism; Metabolic pathways; Pentose and glucuronate interconversions; Starch and sucrose metabolism
• Function: NAD or NADH binding; UDP-glucose 6-dehydrogenase activity; binding; electron carrier activity; oxidoreductase activity