Recombinant Human Coactosin-Like 1 (Dictyostelium), His-tagged COTL1-3377H

Recombinant Human Coactosin-Like 1 (Dictyostelium), His-tagged

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Recombinant Human Coactosin-Like 1 (Dictyostelium), His-tagged

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Cat. No. : COTL1-3377H
Product Overview : Recombinant human COTL1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 18.1 kDa (162aa).
Description : COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 Binds to F-actin in a calcium-independent manner and has no direct effect on actin depolymerization.
Source : E.coli.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
Purity : > 90% by SDS - PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1t2l. :

Gene Name : COTL1 coactosin-like 1 (Dictyostelium) [ Homo sapiens ]
Synonyms : CLP; FLJ43657; MGC19733; coactosin-like 1; coactosin-like 1 (Dictyostelium)
Gene ID : 23406
mRNA Refseq : NM_021149
Protein Refseq : NP_066972
MIM : 606748
UniProt ID : Q14019
Chromosome Location : 16q24.1
Function : actin binding; protein binding

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