Active Recombinant Human CPA2 protein, His-tagged

• Cat.No.: CPA2-3174H
• Product Overview: Recombinant Human CPA2 protein(NP_001859.1)(Met1-Tyr417), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Tyr417
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH, in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-1.
  The specific activity is >4,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human CPA2 existing as the proform consists of 412 amino acids and has a predicted molecular mass of 46 kDa also as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CPA2 carboxypeptidase A2 (pancreatic) [ Homo sapiens ]
• Official Symbol: CPA2
• Synonyms: CPA2; carboxypeptidase A2 (pancreatic); carboxypeptidase A2
• Gene ID: 1358
• mRNA Refseq: NM_001869
• Protein Refseq: NP_001860
• MIM: 600688
• UniProt ID: P48052