Active Recombinant Human GFRA3 protein, His-tagged

• Cat.No.: GFRA3-3153H
• Product Overview: Active Recombinant Human GFRA3 protein(O60609-1)(Asp32-Trp382) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 32-382 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human GFRA3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Label-free epitope binning assays of monoclonal antibodies enable the identification of antigen heterogeneity (2012)

Gene Information

• Gene Name: GFRA3 GDNF family receptor alpha 3 [ Homo sapiens ]
• Official Symbol: GFRA3
• Synonyms: GFRA3; GDNF family receptor alpha 3; GDNF family receptor alpha-3; GFRa 3; GFR-alpha-3; GDNFR-alpha-3; GPI-linked receptor; GDNF receptor alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3
• Gene ID: 2676
• mRNA Refseq: NM_001496
• Protein Refseq: NP_001487
• MIM: 605710
• UniProt ID: O60609