||Recombinant human CTNNBIP1, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.
||CTNNBIP1 binds CTNNB1 and prevents interaction between CTNNB1 and TCF (T-cell transcription factor) family members. This protein is a negative regulator of the Wnt signaling pathway. Two transcript variants encoding the same protein have been found for this gene.
||MGSSHHHHHH SSGLVPRGSH MNREGAPGKS PEEMYIQQKV RVLLMLRKMG SNLTASEEEF LRTYAGVVNS QLSQLPPHSI DQGAEDVVMA FSRSETEDRR Q.
||11.3kDa (101a) confirmed by MALDI-TOF.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl.
||> 90% by SDS – PAGE.
||1mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.