|Cat. No. :
|Product Overview :
||Recombinant human CBR4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||CBR4 belongs to the short-chain dehydrogenase/reductase family. The formation of heteroteramer with HSD17B8 has NADH-dependent3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones. It plays a role in biosynthesis of fatty acids in mitochondria and has broad substrate specificity and reduces 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro).
||MGSSHHHHHH SSGLVPRGSH MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL.
||> 95% by SDS – PAGE.
||27.5kDa (257aa) confirmed by MALDI-TOF.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 5mM DTT, 200mM NaCl.
||0.5mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.