Recombinant Human TIMP Metallopeptidase Inhibitor 2

• Cat.No.: TIMP2-2887H
• Product Overview: Recombinant Human TIMP2 is expressed in Sf9 cells. TIMP-2 shows a protein band with a molecular weight of 21-kDa (nonreduced) or 24-kDa (reduced) on SDS-polyacrylamide gel electrophoresis.

Specification

• Cat. No.: TIMP2-2887H
• Description: The tissue Inhibitor of Metalloproteinases (TIMP) belongs to a family which takes part in the activation and regulation of the MMP-activity: TIMP-1, a 28-kDa glycoprotein; TIMP-2, a 21-kDa glycoprotein and the N-glycosylated TIMP-3 (27-kDa, reduced SDS-PAGE). TIMP-4 is thought to function in a tissue-specific fashion in the ECM hemostasis. These proteins are expressed in a variety of cell types and form with latent as well as with active MMPs non-covalent, stoichiometric complexes. Progelatinase B and the activated gelatinase B specifically bind TIMP-1 via their C-terminal domain. Purified TIMP-2 inhibits the activities of stromelysin 1 (MMP-3), matrilysin (MMP-7), gelatinase A (MMP-2) and the interstitial collagenase (MMP-1).On the MMP-2 the TIMP displays two distinct binding sites.
• Source: Sf9 cells.
• Form: Liquid, in 50 mM Tris-HCl, pH 7; 200 mM NaCl, 5 mM CaCl2, 1 μM ZnCl2, 0.05% Brij 35, 0.05% NaN3.
• Purity: homogenous by electrophoresis and western blotting analysis.
• Specific Activity: It is recommended to perform a pre-incubation of about 20 min 37°C which allows the formation of the enzyme-inhibitor complex.
• Stability And Storage: The protein can be kept stored at -70°C for month, at -20°C for several weeks and at 4°C for 1 week without significant loss of inhibitory activity. Repeated freezing and thawing must be avoided.

Gene Information

• Gene Name: TIMP2 TIMP metallopeptidase inhibitor 2 [ Homo sapiens ]
• Synonyms: TIMP2; TIMP metallopeptidase inhibitor 2; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP-2
• Gene ID: 7077
• mRNA Refseq: NM_003255
• Protein Refseq: NP_003246
• MIM: 188825
• UniProt ID: P16035
• Chromosome Location: 17q25
• Function: enzyme activator activity; enzyme inhibitor activity; integrin binding; metalloendopeptidase inhibitor activity; protein binding