Recombinant E. coli Holliday Junction DNA Helicase RuvA RuvA-2664E

Recombinant E. coli Holliday Junction DNA Helicase RuvA

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Recombinant E. coli Holliday Junction DNA Helicase RuvA

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Cat. No. : RuvA-2664E
Product Overview : Recombinant E. coli Holliday Junction DNA Helicase RuvA is expressed in E. coli. MW= 22 KDa.
Description : E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination pair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
Source : E. coli.
Form : 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
Purity : RuvA protein over 90% by SDS-PAGE (CBB staining).
Usage : 1) Studies on homologous recombination mechanism. 2) For SNP analysis. 3) Incorporation to DNA circuit. 4) Recognition and identification of cross-like DNA.
Concentration : 2.7 mg/ml (determined by BCA method).
Storage : -200C.
Gene Name : ruvA Holliday junction DNA helicase RuvA [ Escherichia coli UTI89 ]
Synonyms :
ruvA; Holliday junction DNA helicase RuvA
Gene ID : 3989992
Protein Refseq : YP_541072

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