Recombinant E. coli Holliday Junction DNA Helicase RuvA

• Cat.No.: RuvA-2664E
• Product Overview: Recombinant E. coli Holliday Junction DNA Helicase RuvA is expressed in E. coli. MW= 22 KDa.

Specification

• Cat. No.: RuvA-2664E
• Description: E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination pair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
• Source: E. coli.
• Form: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
• Purity: RuvA protein over 90% by SDS-PAGE (CBB staining).
• Usage: 1) Studies on homologous recombination mechanism. 2) For SNP analysis. 3) Incorporation to DNA circuit. 4) Recognition and identification of cross-like DNA.
• Concentration: 2.7 mg/ml (determined by BCA method).
• Storage: -20⁰C.

Gene Information

• Gene Name: ruvA Holliday junction DNA helicase RuvA [ Escherichia coli UTI89 ]
• Synonyms:
  ruvA; Holliday junction DNA helicase RuvA
• Gene ID: 3989992
• Protein Refseq: YP_541072