||Recombinant E. coli Holliday Junction DNA Helicase RuvA is expressed in E. coli. MW= 22 KDa.
||E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination pair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
||50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
||RuvA protein over 90% by SDS-PAGE (CBB staining).
||1) Studies on homologous recombination mechanism. 2) For SNP analysis. 3) Incorporation to DNA circuit. 4) Recognition and identification of cross-like DNA.
||2.7 mg/ml (determined by BCA method).