||Recombinant rat Tnfsf11 is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues). It is expressed inE. coli.
||RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a molecule important in bone metabolism. This natural and necessary surface-bound molecule found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Overproduction of RANKL is implicated in a variety of degenerative bone diseases, such as rheumatoid arthritis and psoriatic arthritis.
||Greater than 98% (determined by SDS-PAGE and HPLC analysis).
||< 0.1 ng per μg of rHu APO-E4 (1EU/μg).
||Lyophilized from a sterile-filtered solution with no additives.
||Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
||The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.