||Recombinant Human Nitric Oxide Synthase 3 (Endothelial Cell) is producedinSf9 cells.
||Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase, but also denotes the human gene encoding it. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells. Some association between a polymorphism in the gene and late-onset Alzheimer"s disease in Chinese have been reported.
||Liquid. In 50mM triethanolamine (pH 7.5), 0.5mM EDTA, 7mM GSH, 20mM CHAPS, 1µM E64, 1µM leupeptin, 1µM pepstatin A, 1mg/ml trypsin inhibitor and 10% glycerol.
||≥0.1nmol/100μl/min (U/100μl). Amount assayed 1µl. Optimal conditions: 37°C, pH 7.0, 1mM L-arginine, 1mM NADPH, 10µM (6R)-tetrahydrobiopterin, 5µM FAD, 5µM FMN, 50nM calmodulin, 1mM CaCl2 and 7mM GSH.
||-80°C. eNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle.