Recombinant Human Nitric Oxide Synthase 3 (Endothelial Cell)
Cat.No. : | NOS3-635H |
Product Overview : | Recombinant Human Nitric Oxide Synthase 3 (Endothelial Cell) is producedinSf9 cells. |
- Specification
- Gene Information
- Related Products
Cat. No. : | NOS3-635H |
Description : | Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase, but also denotes the human gene encoding it. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells. Some association between a polymorphism in the gene and late-onset Alzheimer"s disease in Chinese have been reported. |
Source : | Sf9 cells. |
Formulation : | Liquid. In 50mM triethanolamine (pH 7.5), 0.5mM EDTA, 7mM GSH, 20mM CHAPS, 1µM E64, 1µM leupeptin, 1µM pepstatin A, 1mg/ml trypsin inhibitor and 10% glycerol. |
Specific Activity : | ≥0.1nmol/100μl/min (U/100μl). Amount assayed 1µl. Optimal conditions: 37°C, pH 7.0, 1mM L-arginine, 1mM NADPH, 10µM (6R)-tetrahydrobiopterin, 5µM FAD, 5µM FMN, 50nM calmodulin, 1mM CaCl2 and 7mM GSH. |
Storage : | -80°C. eNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle. |
Gene Name : | NOS3 nitric oxide synthase 3 (endothelial cell) [ Homo sapiens ] |
Synonyms : | NOS3; nitric oxide synthase 3 (endothelial cell); eNOS; ECNOS; EC 1.14.13.39 |
Gene ID : | 4846 |
mRNA Refseq : | NM_000603 |
Protein Refseq : | NP_000594 |
MIM : | 163729 |
UniProt ID : | P29474 |
Chromosome Location : | 7q36 |
Pathway : | Arginine and proline metabolism; Calcium signaling pathway; Metabolic pathways; VEGF signaling pathway; Metabolism of nitric oxide |
Function : | FAD binding; FMN binding; NADP or NADPH binding; actin monomer binding; arginine binding; cadmium ion binding; calmodulin binding; heme binding; nitric-oxide synthase activity; oxidoreductase activity; protein binding; tetrahydrobiopterin binding |
Products Types
◆ Recombinant Protein | ||
NOS3-6138M | Recombinant Mouse NOS3 Protein, His (Fc)-Avi-tagged | +Inquiry |
Nos3-4462M | Recombinant Mouse Nos3 Protein, Myc/DDK-tagged | +Inquiry |
NOS3-3684R | Recombinant Rat NOS3 Protein, His (Fc)-Avi-tagged | +Inquiry |
NOS3-1526H | Recombinant Human NOS3 Protein, His (Fc)-Avi-tagged | +Inquiry |
NOS3-1156H | Recombinant Human NOS3 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
NOS3-1206HCL | Recombinant Human NOS3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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