Deubiquitinase Activity Fluorometric Assay Kit

• Cat.No.: Kit-2103
• Product Overview: Deubiquitinase Activity Assay Kit provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate to detect activity as low as 0.25 µU with purified enzyme.

Specification

• Size: 100 assays
• Applications: Measurement of DUB activity in various tissues/cell extractsDetermination of DUB activity associated with pathological conditionsCharacterization of activity of purified DUB enzymes
• Storage: Store kit at –20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the experiment.DUB Assay Buffer: Ready to use. Warm to room temperature before use. Store at -20°C.1 M DTT: Ready to use. Warm to room temperature before use. Store at -20°C.DUB Substrate and AMC Standard: Ready to use. Warm DUB Substrate and AMC Standard to room temperature before use. Light sensitive. Do not expose components to light for extended periods of time. Store at -20°C. Use within six months.DUB Positive Control: Reconstitute with 22 ul of DUB Assay Buffer with DTT to prepare the stock solution. Aliquot & store at -80°C.Avoid repeated freeze/thaw. Use within two months.