Digest Buffer, Mass Spec Grade

• Cat.No.: Digest Buffer-52

Specification

• Description: Digest Buffer is a BT surfactant-based buffer optimized for proteomics and biopharmaceutical research. It facilitates protein denaturation, reduction, and alkylation, followed by enzymatic hydrolysis to convert proteins into peptides with high efficiency and recovery. The surfactant is then degraded by formic acid, allowing direct peptide analysis via mass spectrometry without the need for desalting, minimizing peptide loss. Ideal for proteomics, biopharmaceuticals, and single-cell research.
• Form: Lyophilized powder with 5 mg BT Surfactant and 48 mg ammonium bicarbonate
• Molecular Mass: 393.28 Da
• Purity: > 99.9 % BT Surfactant peak area, analyzed by Thermo QE HF mass spectrometry coupled with positive ion mode ESI.
• Storage: Powder at -20 centigrade, reconstituted solution at 4 centigrade for 6 months
• CAS No.: 881640-19-3
• Usage Notes: Reference Protocol: Add 100 μL Digest Buffer to 10 μL (50 μg) protein sample and mix well. Add 1 μg MS-grade protease and incubate at 42 centigrade for 40 minutes. Then, add 3 μL formic acid and incubate at 42 centigrade for 20 minutes.
• Resuspension Buffer: Dissolve in 12 mL MS Grade water
• pH Range: pH 7-9 is used for proteolysis experiments, will degrade and precipitate in solution at pH 2-4
• Degradation: 0.1 % BT Surfactant peak area after incubation with 0.2 % (v/v) formic acid at 42 centigrade for 30 minutes, analyzed by QE HF mass spectrometry using negative ion mode ESI.