"DNA-Protein" Related Products

DNA-Protein Binding Fluorometric Assay Kit

Cat.No.: Kit-0305
Product Overview: Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes. DNA-Protein Binding Assay Kit (Fluorometric) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. The kit is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.
Size: 96 tests
Applications: Functional Studies more details
Target Species: Reacts with: Mouse, Human; Predicted to work with: all Mammals
Kit Components: Components: 96 tests; 10X Wash Buffer: 1 x 20ml; 8-Well Assay Strips (with Frame): 12 units; 96-Well Assay Plate: 1 unit; Antibody Buffer: 1 x 6ml; Assay Buffer: 1 x 10ml; DTT (500X): 1 x 22µl; Extract Cleaner: 1 x 100µl; Fluoro Developer: 1 x 0.2ml; Oligo Release Solution: 1 x 11ml
Assay time: 3h 00m

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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