E3 Ligase Auto-Ubiquitylation Assay Kit

• Cat.No.: Kit-5900
• Product Overview: Ubiquitin E3 ligase (EC 2.3.2.B11) carry out the final step in the ubiquitination cascade, catalyzing transfer of ubiquitin from an E2 enzyme to form a covalent bond with a substrate lysine. The covalent attachment of ubiquitin to proteins (ubiquitylation or ubiquitination) plays a fundamental role in the regulation of cellular function through biological events involving cell cycle, differentiation, immune responses, DNA repair, chromatin structure, and apoptosis.
  
  E3 ligases also undergo auto-ubiquitinylation, which can be used to detect ubiquitin E3 ligase activity. Utilizing the first three steps in the ubiquitin cascade, the kit facilitates ubiquitylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied).
  
  Note: The Kit provides sufficient material for approximately 10 auto-ubiquitinylation assays. Hdm2 ubiquitin E3 ligase enzyme is also provided for use as a positive control.

Specification

• Storage: -80°C
• Size: 10 Tests