Enolase-phosphatase E1

• Cat.No.: CBCRY26

Specification

• Source: E.coli
• Tag: Non
• Background: Enolase-phosphatase E1 (MASA) is a bifunctional enzyme in the ubiquitous methionine salvage pathway that catalyzes the continuous reactions of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite using Mg2+ as cofactor. In this study, we have determined the crystal structure of MASA and its complex with a substrate analog to 1.7A resolution by multi-wavelength anomalous diffraction and molecular replacement techniques, respectively.
• Protein Classification: Hydrolase
• Structure Weight: 29316.11 Da
• Polymer: 1
• Molecule: E-1 Enzyme
• Chain Length: 285 amino acids
• PDB ID: 1ZS9
• MMDB ID: 33918
• Method: X-Ray Diffraction
• Resolution: 1.7Å
• Ligand Chemical Component: Selenomethionine
• Reference: Wang, H., Pang, H., Bartlam, M., Rao, Z. (2005) Crystal Structure of Human E1 Enzyme and its Complex with a Substrate Analog Reveals the Mechanism of its Phosphatase/Enolase J.Mol.Biol. 348: 917-926

Gene Information

• Gene Name: ENOPH1
• Synonyms: DKFZp586M0524; E1; FLJ12594; MASA; MST145; E-1 enzyme; Enolase-phosphatase E1; MSTP145 protein; acireductone synthase; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog
• UniProt ID: Q9UHY7
• Gene ID: 58478
• Chromosome Location: 4q21.22
• Function: magnesium ion binding; phosphor-glycolate phosphatase activity; acireductone synthase activity; metal ion binding; catalytic activity