| Cat.No.: | Kit-2385 |
| Product Overview: | Activation of caspases plays a central role in apoptosis. The CaspGLOW;Fluorescein Active Caspase-9 Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in; Living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. |
| Applications: | Sensitive detection of activated caspase-9 in living cells. |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Kit Components: | FITC-ATAD-FMK; Wash Buffer; Z-VAD-FMK |
| Target Species: | Mammalian |
| Detection method: | Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) |
| Features & Benefits: | Simple one-step procedure; takes only 1-2 hours; Fast and convenient; The FITC label allows for direct detection of the activated caspases in apoptotic cells |
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