"Phosphate" Related Products

        Fluorescent Phosphate Assay Kit

        Cat.No. : Kit-0689
        Product Overview : Fluorescent Phosphate Assay Kit is used for assessing phosphate over a wide range of concentrations.
        Description : Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles.One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions. The newly designed Fluorescent Phosphate Assay Kit provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi in a variety of samples after reactions of ATPases, GTPases, 5"-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase. Glucose background can be eliminated by doing a glucose control. Phosphate concentrations between 0.4 µM and 10 µM, with a lower limit of detection of approximately 40 pmol, can be directly determined.
        Target Species : Mammals
        Usage : For research use only (RUO)
        Storage : Store kit at -20°C, protect from light. Allow reagents to warm to room temperature and briefly centrifuge vials before opening.
        Kit Components : 1. Assay Buffer: 25 ml.2. OxiRed probe: 1 vial.3. Dimethylsulfoxide (DMSO; Dried): 0.4 ml.4. Maltose Converter: 1 vial.5. Glucose Developer: 1 vial.6. Substrate: 0.2 ml.7. Phosphate Standard (100mM): 50 µl.
        Detection method : Fluorometric
        Compatible Sample Types : Biological Fluid, Cell Lysate, Culture Medium, Plasma, Serum, Tissue Lysate, Urine

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.