Fumarase Specific Activity Assay Kit

• Cat.No.: Kit-0345
• Product Overview: Fumarase Specific Activity Assay Kit is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme"s functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme"s relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

Specification

• Applications: Sandwich ELISA
• Storage: All components are shipped cold. Upon receipt store 200XFumarate, 200X NAD+, 100X Coupler and 100X Reagent Dye at-20°C or preferably at -80°C for longer stability. Store all othercomponents at 4°C.