Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

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Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

G6PDH Related Products

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Cat.No. : Kit-2119
Product Overview : In the G6PDH assay kit, Glucose-6-Phosphate is oxidized by glucose-6- phosphate dehydrogenase to generate 6-Phosphoglucono-δ-Lactone and form NADH, which reacts with a probe to produce a signal at OD 450 nm. In the presence of G6PDH inhibitor, these reactions are impeded, thus decreasing the rate and/or extent of generation of G6PDHdependent absorbance. Thus, this kit provides a sensitive, quick, and easy method for screening potential inhibitors of G6PDH. G6PDH Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.
Size : 100 assays
Applications : Screening/characterizing/studying potential inhibitors of Glucose-6-Phosphate Dehydrogenase
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read the entire protocol before performing the assay.G6PDH Assay Buffer: Bring to room temperature before use. Store at 4ºC or -20°C.G6PDH Developer: Reconstitute with 220 µl dH2O.Pipette up and down to dissolve completely. Store at -20°C. Use within two months. Keep on ice while in use.G6PDH Substrate: Reconstitute with 220 µl G6PDH Assay Buffer. Store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.G6PDH Enzyme: Reconstitute with 100 µl G6PDH Assay Buffer, mix thoroughly. Aliquot and store at -70°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.G6PDH Inhibitor Control: Reconstitute with 200 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.

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