Glucose Uptake Fluorometric Assay Kit

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Glucose Uptake Fluorometric Assay Kit

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Cat.No. : Kit-2250
Product Overview : Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
Applications : This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
Storage : -20°C
Size : 100 assays

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