Glutamic Oxaloacetic Transaminase Activity Assay Kit
Cat.No. : |
Kit-0381 |
Product Overview : |
Glutamic Oxaloacetic Transaminase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of GOT with a detection limit of 10 mU per well. |
- Specification
- Gene Information
- Related Products
Size : |
100 x 1 assay |
Applications : |
Functional Studies |
Storage : |
Store the kit at -20°C protected from light. Allow the Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.GOT ENZYME MIX: Reconstitute with 220 μL dH2O. Aliquot and store at -20°C. Use within two months.GOT DEVELOPER: Reconstitute with 820 μL dH2O. Aliquot and store at -20°C. Use within two monthsGOT SUBSTRATE: Reconstitute with 1.1 ml assay buffer. Store at -20°C. Use within two months.5GOT POSITIVE CONTROL: Reconstitute with 100 μL dH2O. Aliquotand store at -20°C. Use within two months. In the assay (optional),add 5 μL positive control and adjust the volume to 50 μL/well with Assay Buffer. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.