"GLO-1" Related Products

Glyoxalase I Assay Kit

Cat.No.: Kit-0371
Product Overview: Glyoxalase I Assay Kit is a quantitative colorimetric determination of glyoxalase I at 240 nm.
Description: GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S- lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer.Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. Glyoxalase I Assay Kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S- lactoylglutathione, by measuring the change in absorbance at 240 nm.
Applications: Direct Assays: GLO-1 activity in enzyme preparations or biological samples.Drug Discovery/Pharmacology: effects of drugs on GLO-1 activity.
Usage: For research use only (RUO)
Storage: Store the plate at room temperature and other components at -20°C. Shelf life: 12 months after receipt.
Kit Components: Assay Buffer (pH 6.6) 20 mL96 well UV Titer Plate 1 PlateSubstrate 1 mLCosubstrate 1 mL
Detection method: Colorimetric
Compatible Sample Types: Biological Sample
Features & Benefits: Sensitive and accurate. Detection limit 4 U/L GLO-1 activity.Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate. Can be readily automated as a high-throughput assay for thousands of samples per day

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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