GMP Recombinant Human TNF Protein
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|Product Overview :||Recombinant Human TNF, Val77-Leu233, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Source :||E. coli|
|Form :||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity :||Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
|Molecular Mass :||Predicted Molecular Mass: 17 kDa
SDS-PAGE: 17 kDa, reducing conditions
|N-terminal Sequence Analysis :||Met-Val77- Arg-Ser-Se r-Ser-Arg- Thr-Pro-Se r|
|Endotoxin :||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity :||>97%, by SDS-PAGE with silver staining.|
|Storage :||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution :||Reconstitute at 100-200 μg/mL in PBS.|
|Quality Statement :||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name :||TNF tumor necrosis factor [ Homo sapiens ]|
|Official Symbol :||TNF|
|Synonyms :||TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha;|
|Gene ID :||7124|
|mRNA Refseq :||NM_000594|
|Protein Refseq :||NP_000585|
|UniProt ID :||P01375|
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