GMP Recombinant Human TNF Protein TNF-106HG

GMP Recombinant Human TNF Protein

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GMP Recombinant Human TNF Protein

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Cat.No. : TNF-106HG
Product Overview : Recombinant Human TNF, Val77-Leu233, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Source : E. coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
Molecular Mass : Predicted Molecular Mass: 17 kDa
SDS-PAGE: 17 kDa, reducing conditions
N-terminal Sequence Analysis : Met-Val77- Arg-Ser-Se r-Ser-Arg- Thr-Pro-Se r
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >97%, by SDS-PAGE with silver staining.
Storage : Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100-200 μg/mL in PBS.
Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha;
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375

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