Granzyme B Activity Fluorometric Assay Kit

• Cat.No.: Kit-2268
• Product Overview: Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

Specification

• Tag: Non
• Applications: Measurement of Granzyme B activity in various biological samples or purified Granzyme B activity, Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Granzyme B Assay Buffer; Granzyme B Substrate; Positive Control (Granzyme B Enzyme, human recombinant); AFC Standard (1mM)
• Detection method: Fluorescence (Ex/Em = 380/500 nm)
• Features & Benefits: Simple and Sensitive;
  Rapid and convenient;
  Kit contains all necessary reagents for measuring Granzyme B Activity.