"HAT" Related Products

        HAT Activity Colorimetric Assay Kit

        Cat.No. : Kit-0407
        Product Overview : Simple procedure
        Fast and convenient
        The detection can be continuous and suitable for kinetic studies
        Description : Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient,nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
        Applications : Detects HAT activity by colorimetric method in mammalian samples
        Usage : For Research Use Only! Not For Use in Humans
        Storage : -80°C
        Size : 100 assays
        Kit Components : • 2X HAT Assay Buffer
        • HAT Substrate I
        • HAT Substrate II
        • NADH Generating Enzyme
        • HAT Reconstitution Buffer
        • Nuclear Extract 4 mg/ml
        Target Species : Mammalian
        Detection method : Absorbance (440 nm)
        Compatible Sample Types : Cell and Tissue lysates

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.