"HDAC" Related Products

        HDAC8 Activity Assay Kit

        Cat.No. : Kit-0433
        Product Overview : HDAC8 Activity Assay Kit is a quantitative fluorometric determination of HDAC8 activity.
        Description : Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC8 Activity Assay Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the AFC molecule, which can be detected fluorometrically (Ex/Em = 380/500 nm). The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-8 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.
        Target Species : Mammals
        Usage : For research use only (RUO)
        Storage : Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use.Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.HDAC-8 Positive Control and HDAC-8 Substrate: Upon thawing aliquot and store at -20°C, use within 2 months.Developer and Trichostatin A: Store at -20°C; use within 2 months.HDAC-8 Assay Buffer: Store at 4°C.
        Kit Components : HDAC-8 Assay Buffer. Cap color: WM. 25 mlHDAC-8 Substrate. Cap color: red. 200 µlHDAC-8 Positive Control. Cap color: green. 20 µlAFC Standard (1 mM). Cap color: yellow. 100 µlDeveloper. Cap color: orange. 1 mlTrichostatin A (10 µM). Cap color: blue. 1 ml
        Detection method : Fluorometric
        Compatible Sample Types : Biological Fluid,Cell Lysate, Plasma, Serum, Tissue Lysate

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.