HEK293 cell lysate

• Cat.No.: Lysate-01
• Product Overview: HEK293 cell lysate (negative control)

Specification

• Source: Human HEK293T cell line
• Form: Lysis Buffer:
  Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)
  Component:
  1 vial of 100ug whole cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Applications: WB, IP
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.