"Homocysteine" Related Products

      Homocysteine Assay Kit (Fluorometric)

      Cat.No. : Kit-1021
      Description : Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine (‘free’ homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke. Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (Ex/Em = 658/708 nm). The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 μM homocysteine.
      Applications : Estimation of Homocysteine in various biological samples
      Storage : -20°C
      Shipping : Gel Pack
      Size : 100 assays
      Kit Components : Homocysteine Assay Buffer; Disulfide Reducing Agent (DTT); Homocysteine Enzyme Mix; Fluorogenic Probe Solution; Develop Solution; Homocysteine Disulfide Standard
      Target Species : Mammalian
      Detection method : Fluorescence (Ex/Em = 658/708 nm)
      Features & Benefits : Simple, sensitive and HTP adaptable Protocol;
      Specific for Homocysteine without interference from other thiol based amino acids;
      This kit can detect as little as 5 μM of Homocysteine in a variety of biological samples

      Online Inquiry

      Note: There will be extra charge for optional service!

      Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

      Optional requirements on this protein    +Expand

      Price Inquiry

      Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.