Human Adult Colon Ascending Membrane Lysate

• Cat.No.: Colon Ascending-7H
• Product Overview: Creative Biomart's membrane proteins are prepared from a large variety of documented and compliant quality tissues, including normal and diseased tissues from human and other species.

Specification

• Species: Human
• Tag: Non
• Description: Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.?
• Concentration: 2 mg/ml
• Components: All components may be considered bio-hazardous and may contain unidentified contagious material.
• Shipping: Dry Ice
• Quality control Testing: 1. Protein lysate is verified on SDS-PAGE analysis as a consistent pattern. 2. Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.
• Safety Testing: Effects of Overexposure: N/A Respiratory Protection: Wear masks while handling protein sample. Ventilation: General ventilation. Fume hoods should maintain a minimum face velocity of 100 lfm. Protective Glove: General lab safe impermeable gloves Eye Protection: Wear splash goggles or full-face shield. Keep eyewash station in working order. Handling and Storage: Wear appropriate protective clothing and avoid using sharp material such as needle, blade or broken glasses. Store in cold.Environment Protection: Treat all lab supplies that have contaminated with protein product with 2% bleach for 30 minutes, then autoclave for reusing or through away as biohazards.
• Storage Buffer: HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors
• Storage Instruction: See User's Manual for storage information. Handling and use as infectious material. Work under fume hood. Protect workers and environment. Store product in a safe place. Avoid unauthorized access.
• Applications: Western Blot (10 μg to 20 μg per lane is recommended for a mini gel). Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, tissue specific expression.