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Human Bladder Lysate
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Cat.No. : | Bladder-28H Optional Service: Optional requirements on this protein |
Source : | Human bladder tissue |
Species : | Human |
Concentration : | 2 mg/mL |
Tissue Type : | Bladder |
Shipping : | Dry ice |
Preparation method : | Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Diagnosis : | Normal |
Storage Buffer : | Bladder lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol. |
Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
◆ Lysates
Bladder-5H | Human Bladder Tissue Lysate | +Inquiry |
Bladder-6H | Human Bladder Tumor Tissue Lysate | +Inquiry |
Bladder-34H | Human Bladder Tumor Lysate | +Inquiry |
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