Human Colon Tumor Lysate
Cat.No. : | Colon-99H |
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Source : | Human adult colon |
Species : | Human |
Concentration : | 2 mg/mL |
Tissue Type : | Colon |
Shipping : | Dry ice |
Preparation method : | Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Diagnosis : | Tumor |
Storage Buffer : | Colon Tumor lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol. |
Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
Tag : | Non |
Products Types
◆ Lysates | ||
Colon-79H | Human Colon Cytoplasmic Lysate | +Inquiry |
Colon-80H | Human Colon Diabetic Disease Lysate | +Inquiry |
Colon-17H | Human Colon Tumor Tissue Lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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