Human Jurkat Membrane Lysate

• Cat.No.: Jurkat-256H

Specification

• Species: Human
• Source: Jurkat Cell Line
• Tag: Non
• Concentration: 2 mg/mL or better
• Cell line: Jurkat (Human Acute T cell Leukemia)
• Shipping: Dry ice
• Preparation method: Jurkat (Human Acute T cell Leukemia) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The Jurkat (Human Acute T cell Leukemia) cell was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Jurkat (Human Acute T cell Leukemia) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Jurkat (Human Acute T cell Leukemia) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
• Diagnosis: Tumor
• Storage Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Storage Instruction: Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Applications: Jurkat (Acute T cell Leukemia) cell membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.